www.microbiometimes.com\/why-flow-cytometry-could-be-the-probiotics-enumeration-method-of-tomorrow\/<\/strong><\/a><\/p>\n\n\n\nKvantifiering av bakterier har fastst\u00e4llts i \u00f6ver 100 \u00e5r med en indirekt metod d\u00e4r man odlar bakterier p\u00e5 plattor och sedan avl\u00e4ser antal koloniformande enheter.<\/p>\n\n\n\n
Denna metod har fungerat v\u00e4l och fungerar v\u00e4l som oftast speciellt inom livsmedels-industrin. Metoden inneb\u00e4r att man best\u00e4mmer antal kolonier\/gram eller milliliter som avl\u00e4ses efter en viss tidsrymd ex 2-3 dagar. Olika bakterier kr\u00e4ver olika temperaturer, olika m\u00e4ngd syre och olika substrat p\u00e5 odlingsplattan. Om man endast har en bakteriestam p\u00e5 plattan kan denna avl\u00e4sas v\u00e4l dock med metodfel +\/- 30 % vilket varierar mellan laboratorier och handl\u00e4ggare. Om man har flera olika bakterier p\u00e5 samma platta blir det sv\u00e5rt att f\u00e5 utv\u00e4xt av alla de olika bakterierna d\u00e5 de har interagerar mot varandra. En nackdel \u00e4r att \u00e4ven skadade bakterier kan bilda kolonier om de f\u00e5r optimalt substrat p\u00e5 plattan. Detta inneb\u00e4r att mindre livskraftiga bakterier inkluderas i resultatet. Metodfelet kan d\u00e4rf\u00f6r bli en hel 10-logaritm!<\/p>\n\n\n\n
Probiotiska mj\u00f6lksyraproducerande bakterier analyseras alltj\u00e4mt med denna metod och det fungerar tillfredst\u00e4llande i livsmedel d\u00e4r det \u00e4r l\u00e4gre krav p\u00e5 dos-respons och doseringen \u00e4r ospecifikt. Eftersom de flesta produkter inneh\u00e5ller mer \u00e4n en bakterie-stam \u00e4r det om\u00f6jligt att s\u00e4kerst\u00e4lla kvalitet i de slutliga produkterna och dessutom redovisa b\u00e4st-f\u00f6re datum! Detta inger ingen respekt f\u00f6r konsumenten.<\/p>\n\n\n\n
Vid kliniska studier<\/strong> st\u00e4ller man h\u00f6gre krav p\u00e5 analysmetod f\u00f6r att kunna s\u00e4kerst\u00e4lla dos-respons. D\u00e4rvid anv\u00e4ndes Fl\u00f6des-Cytometri<\/strong> f\u00f6r att s\u00e4kerst\u00e4lla att bakteriecellerna \u00e4r levande och oskadade. Denna analysmetod har anv\u00e4nts de senaste \u00e5ren inom forskningen f\u00f6r att s\u00e4kerst\u00e4lla dos-respons-resultat. Metoden blev ISO-certifierad av ISO Copyright Office<\/em> och International Dairy Federation<\/em> 2014 (ISO\/TC 34\/SC 5).<\/p>\n\n\n\nMetoden l\u00e4mpar sig v\u00e4l f\u00f6r probiotika kosttillskott<\/strong> d\u00e4r kravet p\u00e5 bakteriernas kvalitet \u00e4r st\u00f6rre. Metoden bygger p\u00e5 att bakteriernas cellmembran selektivt sl\u00e4pper igenom olika f\u00e4rg\u00e4mnen. Om membranet \u00e4r skadat sl\u00e4pper det igenom f\u00e4rg\u00e4mne som det inte normalt g\u00f6r. Dessa f\u00e4rg\u00e4mnen f\u00e4rgar in bakteriernas cellk\u00e4rna = arvsmassa<\/p>\n\n\n\nMetoden \u00e4r direkt och en fl\u00f6des-cytometer l\u00e4ser av antalet levande och oskadade eller skadade och d\u00f6da bakterieceller. Metoden l\u00e4ser av alla ing\u00e5ende olika bakterier och totala antalet bakterier och % levande och oskadade fastst\u00e4lls. Metodfelet \u00e4r mindre \u00e4n 10 %!<\/p>\n\n\n\n
Metoden \u00e4r n\u00e5got dyrare \u00e4n odling och de flesta producenter undviker att fastst\u00e4lla totala och i % antalet levande bakterier i sina probiotika produkter s\u00e5v\u00e4l vid produktionstillf\u00e4llet som vid b\u00e4st f\u00f6re datum!<\/em><\/p>\n\n\n\nProbiotika kosttillskott verkar som \u201cdetox\u201d av tarmen och mikrofloran, vilket \u00e4r livsviktigt f\u00f6r magen, h\u00e4lsan, och immunsystemet.<\/strong><\/p>\n\n\n\nLivet finns i det levande!
Levande mat, levande organismer, levande tankar!<\/strong><\/h3>\n","protected":false},"excerpt":{"rendered":"ProBion \u00e4r den enda probiotikan med en adekvat och garanterad kvalitet med mer levande \u00e4n icke-levande bakterier, vilket motsvarar den korrekta definitionen av probiotika. Vi har anv\u00e4nt moderna och innovativa fl\u00f6des-cytometriska analyser sedan 2007! Vi garanterar 90 % levande och oskadade bakterier efter f\u00f6rvaring i rumstemperatur i 3 \u00e5r. Konventionella probiotika saknar denna kvalitets-s\u00e4kring vilket […]<\/p>\n","protected":false},"author":1,"featured_media":5356,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"inline_featured_image":false,"_uag_custom_page_level_css":"","site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[19],"tags":[],"class_list":["post-5355","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-probiotika-for-tarmhalsa"],"uagb_featured_image_src":{"full":["https:\/\/probion.com\/wp-content\/uploads\/2020\/10\/4-weeks-intagram.png",1080,1080,false],"thumbnail":["https:\/\/probion.com\/wp-content\/uploads\/2020\/10\/4-weeks-intagram-150x150.png",150,150,true],"medium":["https:\/\/probion.com\/wp-content\/uploads\/2020\/10\/4-weeks-intagram-300x300.png",300,300,true],"medium_large":["https:\/\/probion.com\/wp-content\/uploads\/2020\/10\/4-weeks-intagram-768x768.png",768,768,true],"large":["https:\/\/probion.com\/wp-content\/uploads\/2020\/10\/4-weeks-intagram-1024x1024.png",1024,1024,true],"1536x1536":["https:\/\/probion.com\/wp-content\/uploads\/2020\/10\/4-weeks-intagram.png",1080,1080,false],"2048x2048":["https:\/\/probion.com\/wp-content\/uploads\/2020\/10\/4-weeks-intagram.png",1080,1080,false],"woocommerce_thumbnail":["https:\/\/probion.com\/wp-content\/uploads\/2020\/10\/4-weeks-intagram-250x250.png",250,250,true],"woocommerce_single":["https:\/\/probion.com\/wp-content\/uploads\/2020\/10\/4-weeks-intagram-325x325.png",325,325,true],"woocommerce_gallery_thumbnail":["https:\/\/probion.com\/wp-content\/uploads\/2020\/10\/4-weeks-intagram-100x100.png",100,100,true]},"uagb_author_info":{"display_name":"Mad","author_link":"https:\/\/probion.com\/sv\/probion-blog\/author\/mad"},"uagb_comment_info":0,"uagb_excerpt":"ProBion \u00e4r den enda probiotikan med en adekvat och garanterad kvalitet med mer levande \u00e4n icke-levande bakterier, vilket motsvarar den korrekta definitionen av probiotika. Vi har anv\u00e4nt moderna och innovativa fl\u00f6des-cytometriska analyser sedan 2007! Vi garanterar 90 % levande och oskadade bakterier efter f\u00f6rvaring i rumstemperatur i 3 \u00e5r. Konventionella probiotika saknar denna kvalitets-s\u00e4kring vilket…","_links":{"self":[{"href":"https:\/\/probion.com\/sv\/wp-json\/wp\/v2\/posts\/5355","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/probion.com\/sv\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/probion.com\/sv\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/probion.com\/sv\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/probion.com\/sv\/wp-json\/wp\/v2\/comments?post=5355"}],"version-history":[{"count":0,"href":"https:\/\/probion.com\/sv\/wp-json\/wp\/v2\/posts\/5355\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/probion.com\/sv\/wp-json\/wp\/v2\/media\/5356"}],"wp:attachment":[{"href":"https:\/\/probion.com\/sv\/wp-json\/wp\/v2\/media?parent=5355"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/probion.com\/sv\/wp-json\/wp\/v2\/categories?post=5355"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/probion.com\/sv\/wp-json\/wp\/v2\/tags?post=5355"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}